Vol. 2, No. 1 (2022)
Table of Contents
Expression Analysis of Drought-Responsive Genes in Oryza sativa L. Using Quantitative Real-Time PCR (qRT-PCR)
Wei Ming Zhang
Abstract: Background: Oryza sativa L. (rice) is an essential food for nearly 3.5 billion people across the world, but drought conditions remain a major threat to its prospects, inflicting significant yield losses of 20–40% in the affected regions. Understanding mechanisms of drought resis...
DNA Barcoding of Indigenous Rice Cultivars Using ITS, matK, and rbcL Gene Sequences
Hiroshi Takeda Nakamura, Kenji Masato Saito, Yuki Haruna Tanaka
Abstract: Background: Oryza sativa L. species have become significant sources of agricultural biodiversity that encompass genetic variation for development and improvement. However, a number of cultivars are morphologically hard to distinguish and taxonomically uncertain. DNA barcoding is a mol...
CRISPR/Cas9-Mediated Gene Editing for Salinity Tolerance in Triticum aestivum L.
Dr. Rajesh Kumar, Dr. Priya Sharma, Dr. Michael Chen, Dr. Fatima Al-Mansouri
Abstract: Background: Salinity of soil is huge limitation on wheat (Triticum aestivum L.) production worldwide and affects over 800 million hectares of cultivated land. Progress in salinity tolerance improvement through traditional breeding has been slow because of polygenic nature of the trait...
Transcriptome (RNA-Seq) Analysis of Heat Stress-Responsive Genes in Zea mays L.
Dr. Rajesh Kumar, Dr. Priya Sharma, Dr. Amit Singh
Abstract: Heat stress is a significant barrier to maize (Zea mays L.) production. Climate change worsens the challenge of ensuring food security. It is important to understand how heat tolerance operates at a molecular level for climate-smart crop development. RNA-Seq based transcriptomic analy...
Metagenomic Characterization of the Rhizosphere Microbiome Associated with Glycine max (L.) Merr. under Organic Farming
A Sharma, R Verma, P Nair, S Deshmukh
Abstract: Soybean (Glycine max (L.) Merr.) ranks among the most economically significant leguminous crops globally and its yield is closely connected to rhizosphere microbiome diversity and function. Organic farming systems are known for the fact that organic inputs are used instead of syntheti...
Isolation and Characterization of Endophytic Bacillus subtilis for Plant Growth Promotion in Zea mays L.
Dr. Ram Singh, Dr. Riya Sahu, Dr. Sumit Pandey
Abstract: The use of endophytic bacteria in agriculture has high potential in achieving sustainability since they improve plant growth as well as ability to withstand adverse conditions. This study is aimed at isolating and studying endophytic Bacillus subtilis bacteria from maize (Zea mays L.)...
Functional Diversity of Arbuscular Mycorrhizal Fungi Associated with Cicer arietinum L. under Water-Limited Conditions
Carlos Javier Moreno, Elena MarÃa Navarro
Abstract: Arbuscular mycorrhizal fungi (AMF) are important in improving drought resistance and nutrient uptake by legumes, but the functional diversity of the AMF community in drought-challenged chickpea has not yet been adequately studied. The objective of this work was to analyze AMF taxa ass...
Loop-Mediated Isothermal Amplification (LAMP) Detection of Xanthomonas oryzae pv. oryzae in Rice
Somchai Anan Prasert
Abstract: Background: Bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae, is a major threat to global rice production, causing significant economic losses, particularly in Asia. Early and accurate detection of the pathogen is critical for effective disease management and preve...
Multiplex PCR Identification of Fusarium graminearum Associated with Wheat Head Blight
Andrew Michael Peterson, Jessica Anne Robinson, Matthew David Cooper, Lauren Michelle Evans, Ryan Christopher Mitchell
Abstract: Background: Fusarium head blight causes so much destruction that it has been given the name wheat head blight. The destruction is caused by a pest called Fusarium graminearum, which causes immense financial damage. It is estimated that the damages amount to over $2 billion. In additio...
Molecular Diversity of Alternaria solani Isolates Using ISSR and RAPD Molecular Markers
Anil Kumar Verma, Priya Meena Sharma, Rakesh Singh
Abstract: Background: Early blight is a common disease in solanaceous plants caused by the fungus Alternaria solani and is responsible for substantial financial losses in agriculture and decreased crop production. It is vital to understand the genetic diversity and population structure of the f...