Vol. 2, No. 2 (2022)
Table of Contents
Genome-Wide Identification of Drought-Responsive Transcription Factors in Pearl Millet (Pennisetum glaucum L.)
Priyanka Meena Verma
Abstract: Background: Pearl millet is categorized as a C4 crop with excellent drought resistance ability and belongs to the cereal crops typically grown in arid and semi-arid areas. Although there is available knowledge regarding transcription factors (TF) as essential regulators of networks of...
Comparative Analysis of microRNA Expression Associated with Salinity Tolerance in Hordeum vulgare L.
Benjamin James Carter, Olivia Rose Mitchell
Abstract: Background: Salinity of soil is one of the main abiotic factors restricting barley (Hordeum vulgare L.) yield worldwide. This phenomenon disrupts ionic and osmotic homeostasis and induces complicated physiological and molecular processes. MicroRNAs (miRNAs) function as post-transcript...
Functional Characterization of WRKY Transcription Factors Regulating Heat Stress Responses in Brassica napus L.
Matthew David Cooper
Abstract: Background: Brassica napus L. is the world's second-largest oilseed crop, and rising ambient and episodic heat events increasingly compromise its flowering, silique set and seed oil yield. Plant-specific WRKY transcription factors are central hubs that couple heat perception to do...
Whole-Genome Resequencing for Genetic Diversity Assessment of Traditional Millet Germplasm
Lukas Andreas Weber, Anna Maria Hoffmann
Abstract: Background: Traditional millets hold great significance in terms of nutrition and climate resilience but still have insufficient genetic variation in comparison to other major cereals and this limits their role in genomic breeding for food and nutrition security. Objective: The aim o...
Isolation and Functional Characterization of Plant Growth-Promoting Paenibacillus polymyxa from Wheat Rhizosphere
Imran Ahmed Siddiqui, Sana Fatima Raza
Abstract: Background: Plant growth-promoting rhizobacteria (PGPR) are increasingly recognized as sustainable alternatives to chemical fertilizers in cereal production. Among these, Paenibacillus polymyxa is notable for its multifunctional plant growth-promoting (PGP) traits and broad-spectrum a...
Diversity Analysis of Endophytic Fungal Communities in Oryza sativa L. Using ITS Metabarcoding
Noah Benjamin Hughes, Emma Victoria Stewart, Lucas Daniel Murphy
Abstract: Background: Endophytic fungi are present in all parts of a plant without showing symptoms and aid the host in growth, nutrient uptake, and resistance against different stresses. In rice (Oryza sativa L.), being one of the major food crops of the world, analyzing these communities is i...
Characterization of Nitrogen-Fixing Diazotrophs Associated with Sugarcane Root Systems
Emily Grace Carter, Olivia Renee Thompson
Abstract: Background: Biological nitrogen fixation (BNF) by root-associated diazotrophs offers a sustainable alternative to synthetic nitrogen fertilizers in sugarcane, a crop with high nitrogen demand and substantial environmental footprint from conventional fertilization. Objective: This stu...
Development of Recombinase Polymerase Amplification (RPA) Assay for Rapid Detection of Ralstonia solanacearum
Clara Sophie Richter
Abstract: Background: Ralstonia solanacearum is a pathogen responsible for bacterial wilt, a disease that affects solanaceous crops. Current methods for diagnosing R. solanacearum infection are slow, taking anywhere from 2 to 7 days for confirmation. Objective: The goal of this study was...
Genetic Diversity Analysis of Puccinia triticina Using Simple Sequence Repeat (SSR) Markers
Anirudh Prakash Singh, Meera Rani Kulkarni, Nisha Kumari Sharma
Abstract: Background: Wheat leaf rust due to Puccinia triticina induces tremendous crop losses all over the globe. It is vital to study the genetic variation of the pathogen for effective resistance breeding and disease management. Objective: The aim of this study is the study of the gen...
High-Resolution Melting Curve Analysis for Identification of Seed-Borne Fungal Pathogens
Li Na Zhao, Hao Tian Zhang, Jian Ming Chen, Xue Fang Wang
Abstract: Background: Seed-borne fungal infecting agents such as Fusarium, and Alternari and Aspergilli species lead to pre-emergent and post-emergent losses and seed quality issues globally. Traditional detection methods are based on morphological culturing techniques and the gel-based PCR met...